Parts

New Parts


Part ID Part Name Short Description
BBa_K5324000 Aprotinin This basic part encodes bovine pancreatic trypsin inhibitor, better known as aprotinin. Aprotinin is a Kunitz-type serine protease inhibitor.
BBa_K5324001 Destructin-1 This part encodes a protein produced by Pseudogymnoascus destructans (P. destructans) and is a serine protease. This has been demonstrated to be the main enzyme that P. destructan uses to degrade collagen and keratin to infect bat hosts.
BBa_K5324002 Destructin-2 This part encodes a protein produced by Pseudogymnoascus destructans (P. destructans) and is a serine protease. This has been demonstrated to be one of six peptidases that P. destructan uses to degrade peptide bonds to infect bat hosts. Shares 90% amino acid identity with Destructin-1.
BBa_K5324003 Destructin-3 This part encodes a protein produced by Pseudogymnoascus destructans (P. destructans) and is a serine protease. This has been demonstrated to be one of six peptidases that P. destructan uses to degrade peptide bonds to infect bat hosts. Shares 56% amino acid identity with Destructin-1.

New Composite Parts


Part ID Part Name Short Description
BBa_K5324011 Aprotinin Secretion Device This composite part produces and secretes aprotinin, a serine protease inhibitor using a C-terminal Hly-A signal peptide.
BBa_K5324012 SPINK8 Secretion Device This composite part produces and secretes SPINK6, a serine protease inhibitor using a C-terminal Hly-A signal peptide.
BBa_K5324013 SPINK6 Secretion Device This composite part produces and secretes SPINK6, a serine protease inhibitor using a C-terminal Hly-A signal peptide.
BBa_K5324014 WAP-4 Secretion Device This composite part produces and secretes Wap-4, a serine protease inhibitor using a C-terminal Hly-A signal peptide.
BBa_K5324017 TEVp Secretion Device This composite part produces and secretes TEVp, a highly specific cysteine protease using a C-terminal Hly-A signal peptide.
BBa_K5324015 Destructin-1 Expression Device This composite part produces Destructin-1, a collagen degrading serine protease normally produced by P. destructans.

Used Parts


Part ID Part Name Short Description
BBa_K4165010 SPINK8 Encoder Encodes for SPINK8, a serine protease inhibitor which can inhibit trypsin-like proteases.
BBa_K4165078 SPINK6 Encoder Encodes for SPINK6, a serine protease inhibitor which can inhibit serine peptidases.
BBa_K4165008 WAP-4 Encoder Encodes for WAP-4, a serine protease inhibitor which can inhibit trypsin-like proteases.
BBa_K554002 HIy-A Secretion Signal Peptide Used to target proteins for secretion using the Type 1 secretion pathway of gram-negative bacteria.
BBa_K112703 Histidine Affinity Tag Used for protein purification to determine if our protein was expressed.
BBa_K243004 Short Linker Sequence Gly-Gly-Ser-Gly used to attach a histidine tag to the protein to prevent interference.
BBa_K3102012 TEV Protease A highly specific cysteine protease that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) and cleaves between the Gln and Gly/Ser residues.
BBa_K3102041 TEV Peptide Signal Peptide signal that is used by TEVp to cleave a specific Gln and Gly residue.
BBa_J23100 Anderson Promoter Constitutive promoter changeable with family members to alter expression rate.
BBa_B0034 RBS Initiates translation of the existing mRNA molecule from the promoter step.
BBa_B0015 Stop Codon A double terminator.