Part ID | Part Name | Short Description |
BBa_K5324000 | Aprotinin | This basic part encodes bovine pancreatic trypsin inhibitor, better known as aprotinin. Aprotinin is a Kunitz-type serine protease inhibitor. |
BBa_K5324001 | Destructin-1 | This part encodes a protein produced by Pseudogymnoascus destructans (P. destructans) and is a serine protease. This has been demonstrated to be the main enzyme that P. destructan uses to degrade collagen and keratin to infect bat hosts. |
BBa_K5324002 | Destructin-2 | This part encodes a protein produced by Pseudogymnoascus destructans (P. destructans) and is a serine protease. This has been demonstrated to be one of six peptidases that P. destructan uses to degrade peptide bonds to infect bat hosts. Shares 90% amino acid identity with Destructin-1. |
BBa_K5324003 | Destructin-3 | This part encodes a protein produced by Pseudogymnoascus destructans (P. destructans) and is a serine protease. This has been demonstrated to be one of six peptidases that P. destructan uses to degrade peptide bonds to infect bat hosts. Shares 56% amino acid identity with Destructin-1. |
Part ID | Part Name | Short Description |
BBa_K5324011 | Aprotinin Secretion Device | This composite part produces and secretes aprotinin, a serine protease inhibitor using a C-terminal Hly-A signal peptide. |
BBa_K5324012 | SPINK8 Secretion Device | This composite part produces and secretes SPINK6, a serine protease inhibitor using a C-terminal Hly-A signal peptide. |
BBa_K5324013 | SPINK6 Secretion Device | This composite part produces and secretes SPINK6, a serine protease inhibitor using a C-terminal Hly-A signal peptide. |
BBa_K5324014 | WAP-4 Secretion Device | This composite part produces and secretes Wap-4, a serine protease inhibitor using a C-terminal Hly-A signal peptide. |
BBa_K5324017 | TEVp Secretion Device | This composite part produces and secretes TEVp, a highly specific cysteine protease using a C-terminal Hly-A signal peptide. |
BBa_K5324015 | Destructin-1 Expression Device | This composite part produces Destructin-1, a collagen degrading serine protease normally produced by P. destructans. |
Part ID | Part Name | Short Description |
BBa_K4165010 | SPINK8 Encoder | Encodes for SPINK8, a serine protease inhibitor which can inhibit trypsin-like proteases. |
BBa_K4165078 | SPINK6 Encoder | Encodes for SPINK6, a serine protease inhibitor which can inhibit serine peptidases. |
BBa_K4165008 | WAP-4 Encoder | Encodes for WAP-4, a serine protease inhibitor which can inhibit trypsin-like proteases. |
BBa_K554002 | HIy-A Secretion Signal Peptide | Used to target proteins for secretion using the Type 1 secretion pathway of gram-negative bacteria. |
BBa_K112703 | Histidine Affinity Tag | Used for protein purification to determine if our protein was expressed. |
BBa_K243004 | Short Linker | Sequence Gly-Gly-Ser-Gly used to attach a histidine tag to the protein to prevent interference. |
BBa_K3102012 | TEV Protease | A highly specific cysteine protease that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) and cleaves between the Gln and Gly/Ser residues. |
BBa_K3102041 | TEV Peptide Signal | Peptide signal that is used by TEVp to cleave a specific Gln and Gly residue. |
BBa_J23100 | Anderson Promoter | Constitutive promoter changeable with family members to alter expression rate. |
BBa_B0034 | RBS | Initiates translation of the existing mRNA molecule from the promoter step. |
BBa_B0015 | Stop Codon | A double terminator. |